Extracellular vesicle isolation and characterization: Toward clinical application

R Xu; DW Greening; HJ Zhu; N Takahashi; RJ Simpson

Journal article

Extracellular vesicle isolation and characterization: Toward clinical application

R Xu, DW Greening, HJ Zhu, N Takahashi, RJ Simpson

Journal of Clinical Investigation | AMER SOC CLINICAL INVESTIGATION INC | Published : 2016

DOI: 10.1172/JCI81129

Abstract

Two broad categories of extracellular vesicles (EVs), exosomes and shed microvesicles (sMVs), which differ in size distribution as well as protein and RNA profiles, have been described. EVs are known to play key roles in cell-cell communication, acting proximally as well as systemically. This Review discusses the nature of EV subtypes, strategies for isolating EVs from both cell-culture media and body fluids, and procedures for quantifying EVs. We also discuss proteins selectively enriched in exosomes and sMVs that have the potential for use as markers to discriminate between EV subtypes, as well as various applications of EVs in clinical diagnosis.

University of Melbourne Researchers

David Greening Author

Related Projects (1)

EXPANSION OF TGF-BETA-SMAD SIGNALING NETWORK AND INTRINSIC EPITHELIAL-MESENCHYMAL TRANSITION

The majority of tumor death occurs due to tumor metastasis. Both tumor growth and tumor spread require angiogenesis, which is thought to be ..

Grants

Awarded by National Health and Medical Research Council

Funding Acknowledgements

The authors were supported, in part, by the National Health and Medical Research Council (NHMRC) of Australia: project grants no. 1057741 (to R.J. Simpson and D.W. Greening) and no. 433619 (to H.J. Zhu) and a La Trobe University Leadership RFA grant (to D.W. Greening). This work was also supported by Operational Infrastructure Support Program funding provided by the Victorian Government. R. Xu is supported by a La Trobe University Post Graduate Fellowship. The authors acknowledge the input of Donna Dorow for proofreading this Review.